Linked or semi-linked downstream processing presents substantial improvements to efficiency, Expense, timelines, and facility footprint. Our flexible portfolio lets you end up picking an intensification approach that aligns together with your distinctive approach goals.
By reducing the pH of your solvent inside a cation exchange column, For illustration, a lot more hydrogen ions can be obtained to contend for positions within the anionic stationary section, thereby eluting weakly certain cations.
Sartorius chromatography consumables go over the complete range of separation technologies and methodologies obtainable to accommodate any method and any mo...
Specialized equipment is necessary for an HPLC separation due to substantial pressures and minimal tolerances below which the separation happens.
Reverse phase HPLC is thoroughly utilized in pharmaceutical analysis, wherever the separation of drug compounds from complicated matrices is very important.
In HPLC, the bigger force necessary to pressure the cellular stage and analyte through the tightly packed column is equipped by a pump rather then gravity.
Substantial variety of processes and movement path configurations accessible to layout your individual multi-phase method.
A linked method is more compact and less difficult to control. In this particular webinar, we give an summary on ways to configure the Resolute® BioSC.
Enables simultaneous and continual Procedure of up to 3 chromatography separations. These might be A part of a batch and/or multi-column system
Reverse period HPLC will be the most often utilized variety of HPLC. It utilizes a nonpolar stationary period as well as a polar cell phase. Reverse period HPLC is particularly helpful with the separation of polar and hydrophilic compounds.
Enables whole automation and integration from the VI, and chromatography process management with one skid
Pharmaceuticals seek advice from goods like medicines and drugs which might be used to prevent, treat or cure diseases and Issues.
Other properties that principle of hplc and gc may be analysed incorporate the amounts of glycans, amino acids and peptides. This is certainly reviewed in additional element while in the article ‘Approaches with the Characterisation of Biopharmaceuticals’.
Gas chromatography (GC) and superior-effectiveness liquid chromatography (HPLC) are both commonly utilized analytical strategies in chemistry. The most crucial difference between the two lies during the cellular phase employed for separation. In GC, the cellular phase is usually a more info gas, ordinarily helium or nitrogen, while in HPLC, it is a liquid solvent. This difference impacts the types of compounds which can be analyzed by Every method. GC is more appropriate for volatile and semi-unstable compounds, as they may be vaporized and carried with the column with the gas.